FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_6331.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_6331.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394234
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA27950.036.831886145
GCTATAT142400.036.299194145
GTAGATA166400.026.314861145
AGTGTAT157600.019.780155145
TCGTGTT81750.017.647484145
CGTATCA138150.012.961746145
TCTCGGG54650.012.867643145
CGTGTAT49850.012.65236145
GCCGTAT164600.012.332344145
CGGTGGG65450.012.295064145
GTGGTCG275150.012.199133145
CGGGGTT118450.012.1974181
TATAGTT200200.012.05862145
TCGGTGG367650.011.949658145
TGGTCGC246500.011.88179145
TAGTGTT139800.011.356759145
CGCCGTA159800.011.296399145
GGTCGCC236450.011.0070915145
TCGCCGT189750.010.8124145
GGGGATA124700.010.6442592
Produced by FastQC (version 0.11.5)