FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_6214.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_6214.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17950571
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA20950.030.107203145
CGTGTAT60400.029.527948145
TCGTGTT100950.028.798723145
GTACGTT28050.023.003414145
GTAGATA198700.016.565063145
CGTATTC197000.015.5277598
AACGTAT198050.015.4815186
GAACGTA208050.015.0162825
GATCCTG100300.014.887415
CGCGAGC106750.014.82681
CGTGCGT30400.014.5451718
TCGAGCT30800.013.8861543
ATGACGT135450.013.7530737
CGGGAAC232500.013.1332142
GCGAGCT129950.012.6137452
GGAACGT249900.012.5884994
TTGATCC111550.012.54204753
CGGTGGG63700.012.519542145
TTTGATC161500.012.4399632
CCGGGAA242950.012.3122991
Produced by FastQC (version 0.11.5)