FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_6119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_6119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20495127
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG80450.040.46103145
TCGTGTT101550.025.12924145
CCGTATA27400.017.72723145
GTAGATA167000.017.103947145
AGAGCGG84550.014.919431145
CGTGTAT53150.014.321966145
CGGGGTT146250.011.4172241
GGGGATA145000.010.8539692
CTCTATT246100.010.840588145
GGGATAG133400.010.7590534
GTGGTCG212600.010.195876145
CGTCGTT31500.09.8963375145
CTCGGTG299400.09.879272145
TGGTCGC199300.09.857768145
GGTCGCC193800.09.763451145
TCGGTGG287600.09.704842145
AGGGGAT167800.09.4223962
CCGGGAT57650.09.3188211
CGGGCAT73900.09.136231
CGGGAAC53450.09.0912562
Produced by FastQC (version 0.11.5)