FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_5764.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_5764.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16970207
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG89700.035.23842145
ACGTCCG140600.032.12367145
TCGTGTT136150.027.902044145
CCGTATA48400.025.913324145
CGGTGGG96950.024.30289145
GTAGATA189650.024.274107145
TGTCCTA95400.024.009925
GATCCTG130650.018.8079855
TGATCCT125800.018.6110694
TCTCGGG131350.018.600397145
GTAGATG137850.017.197424145
TGGTGTT231500.017.067421145
GTCCTAC135150.016.7874766
CTACTCT139950.016.781449
TTGATCC142700.016.4589143
GTGTCCT144900.016.4579944
AGAGCGG98100.016.332237145
GCGGTGT172250.016.2705121
CTGGCTC142950.015.9728929
TCCTACT150300.015.8184187
Produced by FastQC (version 0.11.5)