FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_4340.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_4340.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16996015
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA344900.20292992210232808No Hit
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA317060.18654961177664292No Hit
CCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGAC213040.1253470298772977No Hit
GTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTC187530.11033762914424351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA109700.047.5088924
TCACGAG113500.044.9862
AGCAATT132550.042.49027
GCTCAGA105250.042.2863465
CAATTAG161400.037.05179
CGAGCAA155600.036.056645
GAGCAAT164250.034.2467356
GCAATTA177300.033.6880768
GATCCTG70600.031.6227685
CCGTATA19250.031.258562145
GGTTAGA206050.031.1331634
TCAGATT126800.031.0976187
CCTGGCT131700.029.8229681
GGCTCAG156250.028.9015184
CTGGCTC136200.027.7434982
CAGATTG145300.027.3882628
GATCATG43150.025.19795
TGATCCT88250.024.7231834
GAGTGTG157950.024.280504145
GTAGATA106150.023.767355145
Produced by FastQC (version 0.11.5)