FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_341.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_341.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18391863
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT112800.037.85624145
AGATTAT256200.027.10926145
CCGTATA26700.025.79548145
GTAGATA179950.023.89099145
CGGGAAC107950.018.6954571
AACGTAT120100.017.8652046
TGTTAAT225850.017.526848145
CGTATTC124650.017.329678
GGAACGT115550.017.2514484
AGAGCGG125350.017.235474145
AGTGTAT122400.016.880865145
GAACGTA133900.016.511334
ACGTATT141450.015.5783987
GCGTTAG35800.014.9847823
CGTTAGA40250.014.2273494
CGTGTAT58450.013.271822145
ATTAGTT182350.013.199686145
CGAGGGA99500.013.1134148
CCGGGAA168950.012.4180551
TAGTGTT136000.012.314148145
Produced by FastQC (version 0.11.5)