FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_31013.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_31013.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036283
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT275340.16161976177549997No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG266010.15614321504285883No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC235270.13809937296768315No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG231230.13572796366437442No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC223560.1312258078830928No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT213790.1254909888500913No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT208280.12225671527057867No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC195360.11467290135999736No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC192590.11304695983272878No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC171920.10091403154080031No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG170700.10019791288980115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA137550.052.65392145
CCGTATA26350.051.175034145
TCGTGTT65300.045.075302145
CGCTCAG56550.038.9678844
TTACTTC82550.036.353744
TAGTTGT33550.036.2970359
TTGTACT34800.036.092151
TACTTCC86150.035.0869335
TAGCATA45000.034.79339
CGTGTAT83550.034.27492145
GAGTCTA77050.034.0763472
GCTCAGA70400.031.7133885
CTTTTAC97050.031.6439571
TGTACTC38450.031.3133012
AGTCTAA83850.031.2959353
CCGTGTT136000.030.864943145
GCCAGTA50800.030.8216514
ACTTCCT98600.030.8038866
CTCGGCT73800.030.7917161
GTAGATA144600.029.380224145
Produced by FastQC (version 0.11.5)