FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30907.TR1_batch_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30907.TR1_batch_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16408338
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC240120.14634023263050772No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG207420.12641134038072593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTG140200.043.125668145
TGTCCTA105200.038.3120845
TGTGTAT68400.035.294407145
GTAGATA255650.032.75322145
TCGTGTT77250.030.124811145
CGATCTA42050.029.47864
GATCCTG138800.029.2988225
GTGTCCT142500.028.6398184
CTACTCT147150.028.4239469
ATCTATT47250.027.0017286
GGAACGT264600.026.875464
CCTACTC163500.026.3358158
AACGTAT410550.026.0615966
AAGAGTT86300.026.041662145
CGGTGTC158600.025.6546152
TTCGCCG225600.025.547348145
GTCCTAC161000.025.1690396
GTATTCA435550.024.5816639
CGTATTC438450.024.4526638
TGATCCT171150.023.5491184
Produced by FastQC (version 0.11.5)