FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30886.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30886.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16715859
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG184480.11036226137107281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA25300.049.5950362
CTTACGT36750.034.3765561
GCTATAT84050.031.740858145
GATCCTG50750.025.5677075
CGCTCAG27000.025.2371434
CATTGTA161650.023.94827145
GAACGTA239000.023.2634184
AACGTAT239000.023.1723615
CGTATTC240050.022.7687537
GGAACGT217200.020.5268613
CGGGAAC223100.020.274911
GTATTCA271750.020.219878
AGTGTAT63400.019.89613145
GTTACGA39600.019.2207454
TACGACT39700.018.9897926
CGTGTAT30600.018.952963145
ACTTCCT105200.017.8468426
AGTCTAA90250.017.6718793
TACTTCC114550.017.6556595
GTCTAAC87250.017.6135354
Produced by FastQC (version 0.11.5)