FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30886.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30886.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17529923
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA33750.045.968018145
GTAGATA183800.035.853714145
TTCGCCG137650.032.390278145
GAGTGTG233050.028.898972145
TCGTGTT123500.025.006817145
GAGTGTA147900.018.43043145
CGGTGGG93750.017.631096145
TGTCTTG173900.015.925006145
CGTGTAT64700.015.238789145
TCTCGGG103850.013.263645145
TGTCTTA184300.013.098896145
AGAGCGG101100.013.050766145
GTACTCT84300.011.8667383
CGTATCA334600.011.634939145
GTTACGA36200.011.6134734
CTGTGTT207950.011.121096145
GCCGTAT413800.010.967298145
AAGAGTT230750.010.901935145
AGGCGTA52700.010.8661098
TAGTTGT106750.010.6605459
Produced by FastQC (version 0.11.5)