FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30852.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30852.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13215072
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA38500.047.63224
TCGATCT45550.038.192973
AGTGTTA151050.035.899967145
GATCCTG70700.034.1400385
TGTACTC54500.033.800492
TTGTACT56900.031.896011
CTCGATC64500.027.7730142
CGTGTAT102150.025.194347145
TAGTTGT73150.025.1688589
TGATCCT100750.023.5976984
CTATTAG109900.023.3488
ATCTATT81600.022.9175786
CGGTGGG72200.020.88523145
GATGCCT121800.020.5310425
CTGGCTC111400.019.9754839
CCGTGTT140600.019.748158145
GAGTCTA18700.019.3916232
TGGATGC97400.019.2753093
TAGACCT54150.017.9366877
GGTGGAT116150.017.7503831
Produced by FastQC (version 0.11.5)