FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30849.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30849.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18610387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC357270.191973439348682No Hit
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG276510.1485783181188011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAT92500.033.230656145
CCGGGAA220000.031.0222261
CCGTGTT139050.029.144436145
AGTGTTA239250.027.331877145
GAGCCGG66200.024.420904145
GGGAACG290000.024.0470983
CGGGAAC288750.023.9380572
GAACGTA296000.023.5338425
GGAACGT303250.022.994924
AACGTAT306800.022.8710966
CCGTATA41800.022.72008145
ACGTATT317050.022.268157
CGTATTC328350.021.435988
TCTCGGG73200.021.095186145
TCGTGTT147750.019.1851145
CGGTGGG95150.016.838303145
GTATTCA418050.016.8016099
TATTCAC360950.015.9652379
CATTGTA196900.015.500696145
CCATTGT261050.014.857471145
Produced by FastQC (version 0.11.5)