FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30849.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30849.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15105983
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG185900.12306382179829013No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC151380.10021194913300248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA24750.044.810374
GATCCTG86050.042.2035685
AGTGTTA201550.038.487854145
CGTGTAT101900.035.430546145
TCGATCT32250.032.3690223
TGATCCT109150.032.1428154
CTGGCTC115950.029.5698979
CCGTGTT154450.028.445093145
TTTGATC136650.027.9198512
TTGATCC126000.026.9835533
CTCGATC41150.025.7347532
CGGTGGG81200.024.374142145
TCGTGTT93800.023.727842145
AACGTAT377750.023.4300655
CCGGGAA284700.022.7734321
GGGAACG346200.022.5854472
GGAACGT350750.022.259473
CGGGAAC346450.022.2141571
GAGCCGG78250.021.957691145
TCCTGGC159700.021.7410227
Produced by FastQC (version 0.11.5)