FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30848.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30848.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17068372
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA33750.049.62052145
TCGTGTT93550.041.925335145
AGTGTTA240850.037.89677145
CGTGTAT105800.035.700573145
CCGTGTT151800.032.762383145
CGGTGGG87300.025.99281145
GTAGATA218250.022.621216145
TCTCGGG111100.020.294085145
CGTGTAA39050.019.864874145
AGAGCGG107050.017.20165145
CGTCGTT20400.014.925958145
GAGCGTG48650.013.858723145
GAGCCGG177500.013.151662145
GGGGATA112950.012.9001483
TCGTGCG26450.012.60637957
CGCTCAG40250.012.2464124
TACGGAC70900.012.166495
CGTGCGT27000.011.8129898
TGCGCGA43350.011.7050219
GAACGTA35700.011.5737074
Produced by FastQC (version 0.11.5)