FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30844.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30844.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17893036
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA223000.034.883694145
CCGTGTT150300.032.22145145
CGTGTAT105200.032.11425145
CCGTATA48700.029.624548145
TCGTGTT112600.024.209011145
GAACGTA170200.022.1468494
AACGTAT165900.021.977775
CGGTGGG108650.021.619364145
GTAGATA248450.019.63829145
CGTATTC181000.019.6229277
CCGGGAA166350.019.0288851
CGATCTA25100.018.7718814
CGGGAAC206250.018.0933441
ACGTATT210550.017.4204656
TCTCGGG121900.016.652596145
TATTCAC231000.015.972189
GGGAACG247150.015.5241732
GTATTCA239600.015.217378
GATCCTG139450.014.81454855
GGAACGT280050.013.6680123
Produced by FastQC (version 0.11.5)