FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30786.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30786.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13386775
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA179150.13382610822995084No Hit
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC158260.11822115483378186No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG143920.10750909012813019No Hit
GTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATGCAA138340.1033407971673536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG68250.047.0510525
TTACATT55950.038.6327362
TCCTGGC83950.037.7325367
TGTACTC25950.036.895712
TACATTC60300.035.4665073
AGTGTAT30800.035.30726145
GTGACCT65500.035.3071863
CCTGGCT90400.035.201638
TGATCCT90850.034.6285824
TTGTACT27850.034.4130631
AGTGACC40900.034.2273522
CTTTTAC48950.033.670361
GAGTCTA45600.033.5627022
GTTGGTC80400.032.908275
TGACCTA73750.031.7475624
CTCGGCT44700.030.6993271
TTGGTCC89200.030.4743656
CAGTGAC46500.030.4478231
GAGTTGG90850.029.6846353
TACTTCC59050.028.7252755
Produced by FastQC (version 0.11.5)