FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n02_MT_30785.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n02_MT_30785.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13743439
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC257180.18712929129310357No Hit
CGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGTC196320.14284634289860057No Hit
GCGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGT150540.10953590291338287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA43050.048.8308034
TCGATCT48050.039.5293463
GTCCCAC120700.035.0723729
TTGGTCC119200.034.663756
TGTCCTA95800.033.3687935
ATCTATT67400.032.6956986
GTTGGTC135800.030.4258355
GTGACCT110600.030.151883
TGTACTC31100.028.4522512
CTATTAG86250.026.5584898
TTGTACT33250.026.4210931
TGACCTA139800.025.7183744
CTACTCT133100.025.4330449
GAGTTGG171900.024.3760663
AGGATAT38650.024.197609145
CTACTTT162500.022.8843928
ATGCCGT84600.022.8779058
TCGTGTT61000.022.581669145
CATAGCG60200.022.1558695
TCTATTA103600.021.830517
Produced by FastQC (version 0.11.5)