FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19175151
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1245240.6494029694994319No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG324180.16906255392721548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAC85550.016.373762
ATACGAG32800.015.913495
GAACGTA105700.015.6374775
GATCCTG47500.015.5673045
CGATCTA20500.015.5598584
TACGGCG45500.015.4525966
TGCCGTG59600.015.32536145
CAGTCTA58100.014.92388225-29
GGAACGT97700.014.2466754
GTATTCA115850.013.952368
CGAGGGA56650.013.4347088
TGATACG51500.013.3738733
TATTCAC123600.013.136339
GAGCACC63850.012.942864145
CCGGGAA115850.012.7119151
GGTTAGA66200.012.4839964
TGATCCT62950.011.7465754
TTCCCTA109900.011.2781348
TTGATCC65100.011.0254163
TCCCTAC108400.010.8994069
Produced by FastQC (version 0.11.5)