FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_Neg.11.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_Neg.11.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11696061
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT519170.4438844838446037TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTA403830.34527008708316415TruSeq Adapter, Index 1 (97% over 36bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT129850.11102028281145251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG136350.047.05689145
GGATATC53950.029.2958242
TAGTTGT117950.027.5374939
TCTAGTT128700.027.0389827
CTAGTTG122900.026.5456818
CGTCTTG69400.026.325472145
GATATCT61550.026.2670843
ACTCTAG142350.026.0254655
CGGATAT62250.025.0421311
GTACTCT139300.023.5766543
CTCTAGT155050.023.4725156
TATCTTG61200.023.337265
TGTACTC141800.023.0590292
TTGTACT137900.022.9767911
TACTCTA147550.022.5532194
ATACGAC48850.020.6311041
TGTTACT73850.020.4198952
CTGTTAC76450.020.2010691
CACTATA52950.019.4431579
ACTACCC85850.019.085166
Produced by FastQC (version 0.11.5)