FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_Neg.11.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_Neg.11.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9435699
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT258520.27398076178563985TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA175630.1861335339332041TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG144550.053.315365145
CGTCTTG60500.029.958622145
GGATATC43900.027.5799672
ATATCTT42650.025.8384444
TAGTTGT76700.025.1440379
TCTAGTT80400.024.6172317
TATCTTG44250.024.5764885
GATATCT47850.024.091123
CTAGTTG86350.022.2494078
TACTCTA92950.021.1378944
TTGTACT87400.020.9051821
GTACTCT90400.020.8519523
TGTACTC90100.020.5190472
CGGATAT55950.019.6973721
ACTCTAG114550.018.7343435
GTTACGA35150.018.3572034
CTCTAGT117550.017.7627146
TCTTGGC75250.016.3785347
TTACGAC39950.016.151585
TCGCAAC44400.015.1861869
Produced by FastQC (version 0.11.5)