FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_8724.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_8724.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13255186
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG161040.12149207110334023No Hit
CCGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCC148980.11239374536124955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATT53350.050.0096176
CTATTAG69150.045.6063968
TCGATCT55750.044.475893
CGATCTA58350.043.860874
GATCCTG74650.030.884515
TATTAGT107650.030.104889
CTCGATC97250.026.614932
AGCTATG91200.025.279346145
TCTATTA110800.025.1258137
GATCTAT110300.024.3860685
TGTCCTA46300.022.2356975
TAGACCT70450.022.1251457
AACGTAT280300.022.011515
ATTCTGG31700.021.9559426
CGGGAAC267650.021.6448251
GTATTCA289400.021.3938058
CTGGCTC131350.021.0299539
TATTCAC302650.020.6734779
TGTACTC24550.020.0818672
GGAACGT282550.020.0657233
Produced by FastQC (version 0.11.5)