FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_8404.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_8404.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14456653
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT243560.1684760642729683No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC223860.15484912033234802No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT185550.12834921056761894No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC185230.1281278591939642No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT175870.1216533315145629No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC165110.11421039157542205No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT161570.11176169200436643No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG159780.11052350775798521No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA155940.10786729127412824No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG155890.10783270512199469No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT149140.10316357458396491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCAGA46200.041.429275
GCCAGTA44250.039.1588554
CGCTCAG46850.039.152214
CTTGTTA31950.039.0341761
TGCCGTG59650.038.771587145
GAGTCTA63000.038.667862
TACTTCC85350.037.6309855
CGTATGG43600.036.582176145
TTACTTC86900.036.375764
CTTTTAC90300.035.7321931
CTCGGCT59150.035.6718561
GCATCTG68750.035.643257145
TTACGAC35950.035.4943435
ACTTCCT93300.034.5015956
GTTACGA38200.033.403714
GTAGCAT52150.032.947428
TAGCATA53000.032.283019
TTGTGTC30900.031.6757342
AGTCTAA77900.031.4578573
CCAGTAG54200.031.301275