FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_8337.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_8337.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16296290
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC177700.10904322394851836No Hit
CGGGAACGTATTCACCGCAACATTCTGATTTGCGATTACTAGCGATTCCG176880.10854004193592529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG73300.045.10205145
GATCCTG53000.039.259085
CTCCCTG88650.032.713122
GAAACTA96800.031.1567444
AACGTAT402000.031.0917595
GTATTCA410300.030.6036878
TTGAACG44000.030.1526767
GAACGTA427000.029.780764
CGATCTA16200.029.536774
CGCTCAG28350.029.4089034
TCGATCT16100.028.819793
TATTCAC439300.028.6665449
ACGTATT452750.028.0387786
AAGAAAC111000.027.6285512
TGATCCT81100.027.3548684
TCAGATT49250.027.0864642
CGGGAAC355100.026.9113061
CGTATTC472850.026.5553427
GGAACGT380100.025.9404663
CATTGTA204950.025.575596145
Produced by FastQC (version 0.11.5)