FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_8230.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_8230.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18338359
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT307200.16751771518923803No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC301490.16440402328256307No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC264110.14402052004762259No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT258520.1409722647484434No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG251350.1370624274505696No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA249900.13627173510999538No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT238150.12986440062603202No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC237260.12937907912043822No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA228020.1243404603432619No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC222520.12134128249970458No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG219390.11963447765418925No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC219280.11957449409731807No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG219260.11956358799606878No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC211030.11507572733198211No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT210380.11472127904137988No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC191470.10440956031016733No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG189400.10328077883086485No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT185120.1009468731635148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTCC91100.034.0613335
CTTTTAC97250.032.9536251
TTACTTC94950.032.8328484
GTAGCAT52900.032.480618
TAGCATA54250.031.9406599
CTTGTTA43900.031.8759691
CGCTCAG39050.031.1905674
TTGTGTC42850.030.9627022
AGTCTAA115750.030.5659163
GAGTCTA115100.030.4865762
CGCTAGG10050.030.29834
ACTTCCT105250.029.8951196
CCAGTAG58500.029.4956045
TACGACT47250.029.4599556
GTTACGA46850.029.4021474
GAAACTA173750.029.0832544
TCCCTGA173000.028.958133
CTCGGCT50250.028.2807271
TCTAACA124500.028.1846225
AAGAAAC179550.028.0632512
Produced by FastQC (version 0.11.5)