FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_6331.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_6331.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16394234
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATG153750.021.454998145
CGTCTTG49750.019.673105145
TGCCGTG58950.015.372993145
GCCGTCT350500.012.038357145
GGGGATA115050.011.6581393
ATGCCGG40550.011.263722145
CCGTCTT338950.011.229383145
TATGCCG436850.011.2022085145
GGGATAG111700.010.7096084
TCGTATG550150.010.19981145
TCTCGTT60500.010.185815145
ATGCCGT411950.010.10182145
CGTATGC508400.010.067726145
GAACGTA40450.09.8579594
TATCTCG687050.09.83467145
TGCCGTC378050.09.818676145
GGGGAAT172900.09.68721
GTATGCC498350.09.674296145
AGGGGAT135000.09.6668622
TCTCGTA603350.09.612866145
Produced by FastQC (version 0.11.5)