FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_6214.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_6214.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17950571
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATG150200.036.34635145
CGTATGG61700.025.733255145
TGCCGTG91400.025.144838145
TCTCGTT191550.018.31888145
CGTCTTG102350.018.062925145
CGCGAGC104100.017.3429551
ATGCCGG144550.015.397707145
GATCCTG100200.015.0496565
GAGCTCG71550.014.7937748
AACGTAT198600.014.7114926
TCGAGCT30150.014.6681263
GAACGTA207350.014.4752874
CGTGCGT28600.014.1957318
GAAATCG290650.013.943674145
ACACGTA38750.013.84509145
GCGAGCT130700.013.7565862
CGTATTC223000.013.1994848
TTGAACG78550.012.9215327
ATGACGT136650.012.8391967
AAATCTG232200.012.801398145
Produced by FastQC (version 0.11.5)