FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_6119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_6119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20495127
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATG83950.025.649183145
TCTCGTT104500.022.825346145
ATGCCGG70850.022.716995145
CGTATGG56900.020.641466145
CGCATCG202550.019.72228145
TGCCGTG84400.017.78139145
CGTCTTG78450.014.047157145
CTCGTAT256150.011.915863145
GCATCGG81000.011.54629145
GGGATAG132650.011.4775384
GGGGATA145850.011.3832093
GCATCTG213100.011.329182145
ACACGTA53500.010.705602145
AGGGGAT166900.010.4253742
GCCGTCT163500.010.243114145
TCGCGCC104100.010.168102145
CATCTCG370650.09.8779125145
TATGCCG210250.09.655168145
ATCGCGG45200.09.623889145
GTATGCC265700.09.359235145
Produced by FastQC (version 0.11.5)