FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_4340.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_4340.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16996015
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACGAGCAATTAGTATGGGTTAGCTTCACGCGTTACCGCGCTTCCACA346290.20374776087218094No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA305520.1797597848672174No Hit
CCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGAC205160.12071064893741268No Hit
GTCTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTC177830.10463040895174545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCA116150.054.1807564
TCACGAG117100.052.6888282
AGCAATT144200.047.5110367
GCTCAGA99500.041.8247535
CAATTAG173650.041.667419
CGAGCAA167850.040.5159535
GAGCAAT176550.039.0531546
GATCCTG68600.038.3642855
GCAATTA188650.038.353328
GGTTAGA186650.030.686244
TCAGATT121200.029.9683657
CGATCTA16650.028.7391744
TGATCCT91900.028.0064094
GTAGCAT27750.027.954398
CCTGGCT134250.027.4362451
GGCTCAG154750.027.0795694
CAGATTG142000.025.885048
CTGGCTC136050.025.8457432
GCATCTG85850.024.405735145
CTCACGA293350.023.2830581
Produced by FastQC (version 0.11.5)