FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_341.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_341.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18391863
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG65550.030.747522145
GCGCATG126450.027.635431145
TGCCGTG100500.027.629354145
ATGCCGG117700.024.577316145
TCTCGTT175200.023.132137145
CGGGAAC110300.021.0354351
AACGTAT123000.019.9231995
GCATCTG176200.019.256529145
GAACGTA139800.018.3068084
GGAACGT115400.018.1567124
CGCATCG348350.017.544855145
CGTCTTG110950.016.597567145
ACGTATT148200.016.5354486
CGTATTC152500.015.5932747
CGAGGGA90500.015.0606528
CCGGGAA155400.014.137371
CGATCTA19650.013.6516234
CGTGCTA55800.012.73325355
GAGCACC111100.012.202971145
ACACGTA45200.012.190267145
Produced by FastQC (version 0.11.5)