FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_31013.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_31013.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17036283
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG283070.16615713650683075No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC277510.16289351380227718No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT269310.1580802572955615No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC242150.14213781257331778No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT230420.13525250783871107No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC221840.13021619798168413No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG217960.12793870587850648No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC201080.11803044126468198No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC182910.10736496922480096No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG179960.10563337084738497No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT179460.10533987959697547No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC178160.10457680234591078No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT174480.10241670674289692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG114700.049.555695145
CGCTCAG54650.036.349314
TACTTCC84100.034.8275155
TAGCATA44650.034.7489859
TTACTTC86450.033.8806844
GAGTCTA75350.033.772722
CTCGGCT77750.033.6644821
CTTTTAC93900.031.9668521
AGTCTAA80750.031.5140513
GAAACTA163250.031.1315424
GTTACGA39650.030.7186344
GCCAGTA51550.030.0968424
ACTTCCT98400.030.0608236
TCCTCTA98950.029.89479
AAGAAAC172350.029.530312
TGTTACG41350.029.4559753
TTACGAC41650.029.4177065
GCTCAGA69100.029.3776515
AGAAACT175600.029.1900273
GTAGCAT53050.028.9722678
Produced by FastQC (version 0.11.5)