FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30907.TR1_batch_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30907.TR1_batch_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16408338
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC235910.1437744639341291No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT218380.1330908712387568TruSeq Adapter, Index 19 (97% over 38bp)
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG206320.12574094950993817No Hit
CGAGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCC169330.10319753286408409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTATG150750.047.131428145
TGTCCTA95600.041.634785
CGTCTTG75900.035.820473145
CGATCTA41200.030.4432324
CTACTCT138550.029.4083259
GTGTCCT136400.029.3935874
ATCTATT49100.028.4979536
TGCCGTG93750.028.149582145
GATCCTG139650.026.9443865
AACGTAT415450.026.438196
GGAACGT264700.026.211984
CCTACTC158800.026.1597778
CGGTGTC154700.025.776162
GTCCTAC157900.025.6206386
GTATTCA440650.024.9428429
CGTATTC450700.024.4343158
CCGGGAA296800.023.8919321
CTATTAG71150.023.0283858
TGGGCTA106800.022.9451682
GGGAACG319900.022.9354973
Produced by FastQC (version 0.11.5)