FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30886.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30886.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16715859
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG182510.10918373982455822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA25250.048.2377472
CTTACGT35150.037.3350751
CGCTCAG27900.028.064424
TATGATG80150.026.141441145
GATCCTG47600.025.131295
GAACGTA232800.022.8897424
AACGTAT233400.022.7377785
CGTATTC242400.021.8334757
CATTGTA165700.021.745483145
GTTACGA36000.021.5485384
CGTGCGT41400.020.4889378
GTATTCA267050.019.81828
GAGCACC53050.019.67942145
CGGGAAC217750.019.6452541
GTAGCAT53600.019.6126888
GGAACGT213700.019.6092573
TACGACT39650.019.3820276
ACGTGGC37050.018.7853626
TTACGAC41850.018.536435
GTCTAAC87900.017.8156384
Produced by FastQC (version 0.11.5)