FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30886.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30886.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17529923
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATG245800.040.556126145
CGTCTTG95550.018.210262145
TGCCGTG86500.015.338066145
ATGCCGG91550.011.24516145
AACGTAT67200.011.0043746
CGGGAAC84900.010.76036551
GAACGTA68950.010.61998755
CGATCTA19950.010.538824
GCCGTCT813150.010.494009145
GAGTCTA72750.010.2646952
CCGTCTT787900.010.158594145
CGTATTC80300.010.1118818
ACACGTA43900.010.073977145
TATGCCG996300.010.071209145
GTATGCC1067650.010.070427145
GAGCACC123650.09.7916975145
GGGATAG109600.09.7245821
GTCTAAC68200.09.6737294
ATGATCG63400.09.605626145
GGGGAAT197100.09.6010341