FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30852.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30852.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13215072
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT320360.24242016993929355TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA158320.11980260115117042TruSeq Adapter, Index 3 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA38900.045.4761924
TCGATCT43450.037.7103733
GATCCTG70150.036.2763255
CTCGATC62900.026.2802622
GCATCTG90700.026.218258145
ATCTATT76450.025.415556
TGATCCT100200.025.3246924
TGTACTC53450.024.5514052
GCGCATG102400.024.497028145
TTGTACT57050.022.8762781
CTGGCTC118200.021.7746629
CTATTAG118300.020.897878
CGTATGG70250.020.743738145
TGCCGTG131900.020.667137145
GAGTCTA17750.020.0144272
TCCTGGC138700.018.7128057
TAGTTGT73950.017.6471639
CAATTAG53550.017.6004779
CAGATTG64500.017.3102463
CCTGGCT150350.017.0699488
Produced by FastQC (version 0.11.5)