FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30849.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30849.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18610387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCC350910.18855599295167802No Hit
CGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCGATTCCG277360.14903505230707992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAA220450.030.718811
AGCTATG159550.024.355764145
GGGAACG290350.024.2208563
GAACGTA299850.023.4050585
CGGGAAC294400.023.321312
AACGTAT304300.023.038846
GGAACGT308850.022.6995544
ACGTATT319350.022.134417
CGTATTC325600.021.6205848
GTATTCA417300.017.0265039
TATTCAC363700.016.0671759
CCATTGT254300.014.682318145
CGTTAGA132200.014.3687049
CATTGTA191100.014.340519145
ACACACG149350.013.6893344
CACGTGC159450.012.9583777
TCCGGAC215600.012.83186780-84
GACGTAC193250.012.79280890-94
CCGGACT212350.012.64587780-84
TCGTTAG169550.012.6141178
Produced by FastQC (version 0.11.5)