FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30849.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30849.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15105983
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAACGTATTCACCGCGGCGTGCTGATCCGCGATTACTAGCGATTCCG181130.1199061325568816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG112500.047.430622145
GATCCTG92750.045.336615
CGATCTA22850.039.343274
TGATCCT117900.034.927664
CTGGCTC120650.034.0118189
TTTGATC136850.032.740112
TTGATCC131800.030.1990533
TCGATCT31700.027.2159883
CGTCTTG41700.026.948164145
TCCTGGC159750.024.9604557
CGACCTA21350.024.4494764
CCTGGCT167250.024.2746288
CTCGATC38650.023.822682
AACGTAT375600.023.4716135
GGGAACG342650.022.3857332
ATCCTGG191800.022.3017226
GGAACGT345950.022.1302833
CCGGGAA281950.022.1155991
CGGGAAC344550.021.906391
TCGAGCT31350.021.5070973
Produced by FastQC (version 0.11.5)