FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30848.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30848.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17068372
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATG106150.039.135574145
CGTATGG70350.034.111515145
TGCCGTG93300.033.258236145
ATGCCGG95450.032.736965145
TCTCGTT156050.027.736244145
CGTCTTG115200.024.292484145
GCATCTG191250.022.176424145
CGCATCG366700.019.217304145
ACACGTA40650.016.765032145
GCATCGG119000.015.23106145
ATCGCGG52800.014.5548935145
TCGCGTT72450.014.409908145
GAGCACC102250.014.393613145
GTTACGA21650.013.060214
TCGTGCG26650.013.05798157
CGTGCGT25550.013.0527338
ATCCCGA122450.012.8482634
AGGCGTA30100.012.5248178
GATCCCG122200.012.340583
GCGCATA31250.012.295975145
Produced by FastQC (version 0.11.5)