FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30844.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30844.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17893036
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATG181550.036.459797145
TCTCGTT142650.030.138569145
TGCCGTG116700.028.204908145
CGTATGG86200.023.886404145
ATGCCGG134350.023.52819145
CGATCTA24350.022.0326354
AACGTAT159350.019.8821355
CCGGGAA158600.019.5205611
CGTCTTG123900.019.310003145
GAACGTA167950.019.1230584
CGGGAAC198900.016.366332
ACGTATT208100.015.3984777
CGTATTC208100.015.32888
GATCCTG135650.015.2320625
GGGAACG236000.014.4998953
TATTCAC223000.014.2399279
GTATTCA235200.013.8091938
ACGGGCA79600.013.6619234
TCACGGG77400.013.3010862
AAATCTG267600.013.248371145
Produced by FastQC (version 0.11.5)