FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30786.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30786.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13386775
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC167420.1250637289414366No Hit
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA164710.12303934293360425No Hit
CTTACATTCAGTAGCGAGCTTAACCGAATAGGGGAGGCGTAGGGAAACCG159720.11931178345792769No Hit
GTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATGCAA147110.10989203897129816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG67900.049.970485
TTACATT59100.040.237292
TACATTC61650.038.2198833
TCCTGGC89200.037.793537
TGATCCT92700.036.6801034
CCTGGCT94900.035.6763158
AGTGACC40350.035.21722
GTGACCT66100.033.8919143
CTTTTAC50000.032.482261
GAGTCTA40600.032.1431772
CTCGGCT44550.031.0852181
CAGTGAC47000.030.6989481
GCATCTG65300.030.30973145
ATTCAGT83800.030.020626
GTTGGTC80200.030.012435
TGACCTA77050.029.7338984
ACATTCA84650.028.6059884
CTTACAT86000.027.9903221
TTGGTCC87150.027.8683746
TACTTCC61650.027.7534125
Produced by FastQC (version 0.11.5)