FastQCFastQC Report
Tue 4 Dec 2018
HGFWJBGX9_n01_MT_30785.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFWJBGX9_n01_MT_30785.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13743439
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGTTGGTCCCACGCCTTCCCATCCCGAACAGGACCGTGAAACGACTC279600.20344252992282352No Hit
CGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGTC189660.13800039422447322No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT165030.12007911556925453TruSeq Adapter, Index 19 (97% over 38bp)
GCGGTGACCTACTTTCACATGGAAGAACCACACTATCATCGGCGCTGAGT148390.10797152008314657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA42850.052.9580544
TCGATCT50400.043.8742183
GTCCCAC121550.037.5770389
ATCTATT66200.037.2356956
TTGGTCC122150.036.3242386
GTTGGTC133850.033.3115845
TGTCCTA87200.030.762655
GTGACCT110600.030.3505083
CTATTAG87600.029.2973388
GATCCTG107800.026.1619055
TGACCTA137450.025.8457954
GAGTTGG174050.025.7843823
CTCGATC87250.025.4271492
TCTATTA99700.024.6508567
CTACTCT127850.023.533389
TTACATT48950.023.2535172
CTACTTT161450.023.170788
TGTACTC32950.023.1033822
TATTAGT123500.022.7772719
CATAGCG62250.022.2450075
Produced by FastQC (version 0.11.5)