FastQCFastQC Report
Fri 12 Oct 2018
HGFVNBGX7_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFVNBGX7_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420879638
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29306780.6963221157303885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG7421050.085.337058
GCTCGAT7501150.084.386167
AGCAGTG18905700.083.454822
AAGCAGT19054450.083.002931
GGCTCGA2471400.082.495186
CGGCTCG528500.080.706565
CGCTCGA1633600.080.094426
GCAGTGG21594500.073.101563
CAGTGGT22304800.070.768364
GGGCTCG1011350.069.14325
CCGCTCG395500.068.8681645
AGGCTCG625400.065.703365
AGTGGTA24714700.064.0377355
TGGCTCG811300.062.9376685
GAGCTCG597000.061.054925
TCGCTCG555650.060.9869965
GTGGTAT26076650.060.766046
AGCTCGA2129950.060.3467646
GTATCAA26674300.059.2101979
TGCTCGA2983200.057.5967376
Produced by FastQC (version 0.11.5)