FastQCFastQC Report
Fri 12 Oct 2018
HGFVNBGX7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFVNBGX7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420879638
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACACGT5920554514.067096541268171No Hit
CAGCATCA5669570013.470763344460012No Hit
CCTGTGCG4591345910.908928552157708No Hit
TACTCTTC4327824910.282808929806198No Hit
AGTTCGGC407499109.682081602626734No Hit
GTAATCTT405584679.636595201595378No Hit
TCCGGAAG391309279.297415096142048No Hit
ATGAGAAA378196428.985856901920258No Hit
GGGGGGGG172376764.095630779838297No Hit
CAACGCCT69200481.644186930231108No Hit
ACCATAAC62399641.4826005909081303No Hit
TGTGCGGG57184301.3586853541249244No Hit
GTGTATTA43702601.0383633717153122No Hit
ATTACTCG35432960.8418786940697759No Hit
TCCGGAGA17600470.4181829770534064No Hit
GGAATCTC16288980.38702228688003193No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)