FastQCFastQC Report
Fri 12 Oct 2018
HGFVNBGX7_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFVNBGX7_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420879638
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA1229250.017.8196758
GGGACCT997900.017.5605451
TGACGGC1192100.017.4292321
GGGAATG1064400.017.3869921
CTGCGGA920450.017.3768061
CGCTGGA1183100.017.3558651
GCGCGAT940400.017.3331641
CCGGGAT1036900.017.2900181
ATAACGC1106050.017.276361
CCTACCA1231400.017.1953771
TGGACGC1173850.017.107011
CGGACTG1039050.017.036921
ACGAGGA1006950.016.9729961
TGCCAAA1300250.016.9617441
GGGCATC1246800.016.9543461
CGCTTCA1235500.016.9232461
ACTGATG979950.016.9144121
GATGCTA1200900.016.8992141
CTCGAAA1020400.016.8357321
TGGGCGT1016700.016.821591
Produced by FastQC (version 0.11.5)