FastQCFastQC Report
Sat 8 Sep 2018
HGFVLBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFVLBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465061979
Sequences flagged as poor quality0
Sequence length99
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34803260.7483574571035833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT5347600.080.866167
CTCGATG5384250.080.446328
AGCAGTG10986650.079.442492
AAGCAGT11396400.076.760421
CGGCTCG407700.075.4375845
GGCTCGA1906350.074.0725256
CGCTCGA1209150.072.344236
GCAGTGG12932400.067.702673
CAGTGGT13778400.063.4282154
GGGCTCG787000.060.953815
AGGCTCG502450.058.0374375
AGTGGTA15717950.055.7761845
CCGCTCG316150.055.495515
TGCTCGA2172550.054.808036
TCGCTCG434150.053.747115
AGCTCGA1637400.053.528216
GTGGTAT17526350.050.063416
GAGCTCG528100.047.8044055
TGGCTCG728200.046.9801455
GTATCAA18877350.046.0895589
Produced by FastQC (version 0.11.2)