FastQCFastQC Report
Sat 8 Sep 2018
HGFVLBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFVLBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465061979
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACCT1303850.018.2707611
CAATGGA1471800.017.8787888
GACTAAC1333800.017.825841
ACTGATG1225900.017.7382321
GGGAATG1242650.017.6141131
ACGAGGA1218850.017.4865671
CGGACTG1191000.017.3723741
TGACGGC1316550.017.3667431
ATAACGC1205250.017.2445561
GCGCGAT986350.017.1380711
ACGAGCC1284500.017.0974861
ACTGAAC1217700.017.076871
GACTGCG1276100.017.0626621
GGGGGGC489250.017.06140521
GGGCATC1290200.017.0252081
GACCTGG1268100.017.0145511
TGCGTGG1406050.016.992051
CCGGTAG1297400.016.8708021
TGGGCGT1161250.016.8285831
CGCTGGA1104300.016.8249951
Produced by FastQC (version 0.11.2)