FastQCFastQC Report
Thu 7 Jul 2022
HGFNTBGXM_n01_AKM389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFNTBGXM_n01_AKM389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12347024
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT9129937.394437720376991TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT258400.2092812000689397TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTAT142750.11561490444984962TruSeq Adapter, Index 20 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTAGCTCGTAT141520.11461871297893322TruSeq Adapter, Index 20 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)