FastQCFastQC Report
Tue 17 Aug 2021
HGFHNDRXY_n01_glutamate.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFHNDRXY_n01_glutamate.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126416516
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGAGAACATCTCGTA2970680.23499144684544226TruSeq Adapter, Index 15 (97% over 36bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2883670.22810864365222658No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950120.15426148906049586No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGAGAACATCGCGTA1512450.11964022169381727TruSeq Adapter, Index 15 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)