FastQCFastQC Report
Tue 17 Aug 2021
HGFHNDRXY_n01_glr4mutant3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFHNDRXY_n01_glr4mutant3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119718550
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3514850.29359276402863216No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTA3174220.26514019757172136TruSeq Adapter, Index 18 (97% over 37bp)
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1966180.16423352939039104No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCGCGTA1576310.1316679829483401TruSeq Adapter, Index 18 (97% over 37bp)
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1419440.1185647504083536No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)