FastQCFastQC Report
Tue 17 Aug 2021
HGFHNDRXY_n01_glr4mutant2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFHNDRXY_n01_glr4mutant2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109037597
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3813980.349785771599497No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2549200.23379091892496492No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1769640.16229631326156244No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCCTAGTATCTCGTA1343440.12320887812668872TruSeq Adapter, Index 18 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240180.11373875013037933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)