FastQCFastQC Report
Tue 17 Aug 2021
HGFHNDRXY_n01_col2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGFHNDRXY_n01_col2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116721322
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3525510.30204507107964385No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840350.24334457075460472No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1574400.13488538109600917No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1367490.11715854280677183No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTCGTA1184090.10144590377411936TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)